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Journal Article

Identification of Escherichia coli DNA helicase I as the traI gene product of the F sex factor

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Abdel-Monem,  Mahmoud
Max Planck Institute for Medical Research, Max Planck Society;

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Taucher-Scholz,  Gisela
Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Abdel-Monem, M., Taucher-Scholz, G., & Klinkert, M.-Q. (1983). Identification of Escherichia coli DNA helicase I as the traI gene product of the F sex factor. Proceedings of the National Academy of Sciences of the United States of America, 80(15), 4659-4663. doi:10.1073/pnas.80.15.4659.


Cite as: http://hdl.handle.net/21.11116/0000-0003-5907-2
Abstract
Active DNA helicase I (Mr 180,000) can be isolated from Escherichia coli F+ strains but not F- strains. The transfer of the F sex factor to F- strains by conjugation permits the purification of the enzyme from the transconjugant strains. We conclude from this that helicase I is coded for by a portion of the F factor. Results also obtained by using recombinant plasmids carrying different DNA fragments of the F factor transfer region suggest that DNA helicase I is identical to the product of traI, one of the transfer genes of the F factor.