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Volume EM Reconstruction of Spinal Cord Reveals Wiring Specificity in Speed-Related Motor Circuits

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Svara,  Fabian
Department: Electrons-Photons-Neurons / Denk, MPI of Neurobiology, Max Planck Society;
Department of Computational Neuroethology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

Kornfeld,  Joergen
Department: Electrons-Photons-Neurons / Denk, MPI of Neurobiology, Max Planck Society;

Denk,  Winfried
Department: Electrons-Photons-Neurons / Denk, MPI of Neurobiology, Max Planck Society;

Bollmann,  Johann H.
Department: Electrons-Photons-Neurons / Denk, MPI of Neurobiology, Max Planck Society;

Fulltext (public)

PIIS2211124718307563.pdf
(Publisher version), 4MB

Supplementary Material (public)
Citation

Svara, F., Kornfeld, J., Denk, W., & Bollmann, J. H. (2018). Volume EM Reconstruction of Spinal Cord Reveals Wiring Specificity in Speed-Related Motor Circuits. Cell Reports, 23(10), 2942-2954. doi:10.1016/j.celrep.2018.05.023.


Cite as: http://hdl.handle.net/21.11116/0000-0003-597E-D
Abstract
Spinal interneurons coordinate the activity of motoneurons to generate the spatiotemporal patterns of muscle contractions required for vertebrate locomotion. It is controversial to what degree the orderly, gradual recruitment of motoneurons is determined by biophysical differences among them rather than by specific connections from presynaptic interneurons to subsets of motoneurons. To answer this question, we mapped all connections from two types of interneurons onto all motoneurons in a larval zebrafish spinal cord hemisegment, using serial block-face electron microscopy (SBEM). We found specific synaptic connectivity from dorsal but not from ventral excitatory ipsilateral interneurons, with large motoneurons, active only when strong force is required, receiving specific inputs from dorsally located interneurons, active only during fast swims. By contrast, the connectivity between inhibitory commissural interneurons and motoneurons lacks any discernible pattern. The wiring pattern is consistent with a recruitment mechanism that depends to a considerable extent on specific connectivity.