English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Characterization of a human genomic DNA fragment coding for a myosin heavy chain

MPS-Authors
/persons/resource/persons231978

Appelhans,  Heribert
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95819

Vosberg,  Hans-Peter
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Appelhans, H., & Vosberg, H.-P. (1983). Characterization of a human genomic DNA fragment coding for a myosin heavy chain. Human Genetics, 65(2), 198-203. doi:10.1007/BF00286664.


Cite as: https://hdl.handle.net/21.11116/0000-0003-59D0-E
Abstract
A DNA segment from the human genome with information for myosin heavy chain (MHC) was isolated from a human genomic DNA library cloned in lambda Charon 4A phages. The isolation was accomplished by a myosin cDNA probe obtained from rabbit heart muscle mRNA (Sinha et al. 1982). The selected human DNA clone, designated lambda gMHC1, contains a genomic DNA fragment of about 14 kilobase pairs. The transcriptional polarity of this DNA was determined. The 5'-end of the gene is missing from the cloned fragment. This human gene exhibits sequence homology to MHC DNA of rabbit and chicken, but not to an MHC sequence of nematode. The isolated gene fragment is a member of the human MHC multi-gene family, which is presumed to consist of probably more than ten separate sarcomeric MHC genes per haploid genome.