MHz data collection of a microcrystalline mixture of different jack bean 
proteins

Grünbein, Marie Luise; Bielecki, Johan; Gorel, Alexander; Stricker, Miriam; Bean, Richard; Cammarata, Marco; Dörner, Katerina; Fröhlich, Lars; Hartmann, Elisabeth; Hauf, Steffen; Hilpert, Mario; Kim, Yoonhee; Kloos, Marco; Letrun, Romain; Messerschmidt, Marc; Mills, Grant; Nass Kovács, Gabriela; Ramilli, Marco; Roome, Christopher M.; Sato, Tokushi; Scholz, Matthias; Sliwa, Michel; Sztuk-Dambietz, Jolanta; Weik, Martin; Weinhausen, Britta; Al-Qudami, Nasser; Boukhelef, Djelloul; Brockhauser, Sandor; Ehsan, Wajid; Emons, Moritz; Esenov, Sergey; Fangohr, Hans; Kaukher, Alexander; Kluyver, Thomas; Lederer, Max; Maia, Luis; Manetti, Maurizio; Michelat, Thomas; Münnich, Astrid; Pallas, Florent; Palmer, Guido; Previtali, Gianpietro; Raab, Natascha; Silenzi, Alessandro; Szuba, Janusz; Venkatesan, Sandhya; Wrona, Krzysztof; Zhu, Jun; Doak, Bruce; Shoeman, Robert L.; Foucar, Lutz; Colletier, Jacques-Philippe; Mancuso, Adrian P.; Barends, Thomas; Stan, Claudiu A.; Schlichting, Ilme

doi:10.1038/s41597-019-0010-0

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MHz data collection of a microcrystalline mixture of different jack bean proteins

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Grünbein, Marie Luise
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Max Planck Research Group Behavioural Neurophysiology (Andreas T. Schaefer), Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons192083

Gorel, Alexander
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons225677

Stricker, Miriam
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons117815

Hartmann, Elisabeth
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons183393

Hilpert, Mario
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons203860

Kloos, Marco
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons183396

Nass Kovács, Gabriela
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons183399

Roome, Christopher M.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons117878

Doak, Bruce
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95345

Shoeman, Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons92933

Foucar, Lutz
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons92083

Barends, Thomas
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Heme and Flavin Enzymes, Max Planck Institute for Medical Research, Max Planck Society;
Molecular chaperones, Max Planck Institute for Medical Research, Max Planck Society;
Photoreceptors, Max Planck Institute for Medical Research, Max Planck Society;
Structural Biology of Elemental Cycles, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95189

Schlichting, Ilme
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;
Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society;
Heme and Flavin Enzymes, Max Planck Institute for Medical Research, Max Planck Society;
Photoreceptors, Max Planck Institute for Medical Research, Max Planck Society;
Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society;
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Grünbein, M. L., Bielecki, J., Gorel, A., Stricker, M., Bean, R., Cammarata, M., et al. (2019). MHz data collection of a microcrystalline mixture of different jack bean proteins. Scientific Data, 6: 18 (2019), pp. 1-6. doi:10.1038/s41597-019-0010-0.

Cite as: https://hdl.handle.net/21.11116/0000-0003-5A72-8

Abstract

We provide a detailed description of a serial femtosecond crystallography (SFX) dataset collected at the European X-ray free-electron laser facility (EuXFEL). The EuXFEL is the first high repetition rate XFEL delivering MHz X-ray pulse trains at 10 Hz. The short spacing (<1 µs) between pulses requires fast flowing microjets for sample injection and high frame rate detectors. A data set was recorded of a microcrystalline mixture of at least three different jack bean proteins (urease, concanavalin A, concanavalin B). A one megapixel Adaptive Gain Integrating Pixel Detector (AGIPD) was used which has not only a high frame rate but also a large dynamic range. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development and for data analysis training for prospective XFEL users.
Design Type(s) protocol testing objective
Measurement Type(s) protein structure data
Technology Type(s) x ray crystallography
Factor Type(s)
Sample Characteristic(s) Canavalia

Machine-accessible metadata file describing the reported data (ISA-Tab format)