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The influence of sodium trichloroacetate on the tryptophan fluorescence of sarcoplasmic reticulum ATPase

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Lüdi,  Hans
Max Planck Institute for Medical Research, Max Planck Society;

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Hasselbach,  Wilhelm
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Lüdi, H., & Hasselbach, W. (1983). The influence of sodium trichloroacetate on the tryptophan fluorescence of sarcoplasmic reticulum ATPase. Biochimica et Biophysica Acta-Biomembranes, 733(2), 479-482. doi:10.1016/0005-2736(83)90066-4.


Cite as: https://hdl.handle.net/21.11116/0000-0003-6392-8
Abstract
The chaotropic anion trichloroacetate quenches the tryptophan fluorescence of the sarcoplasmic reticulum calcium transport ATPase. Half-maximum quenching was observed at 50 mM trichloroacetate. In contrast to native preparations, in trichloroacetate-treated sarcoplasmic reticulum vesicles a decrease of the tryptophan fluorescence is observed on addition of millimolar concentrations of calcium. It is concluded that trichloroacetate renders the tryptophan fluorescence of the ATPase sensitive to the occupancy of its low-affinity sites.