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Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis

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Faulstich,  Heinz
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

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Trischmann,  Heinrich
Max Planck Institute for Medical Research, Max Planck Society;

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https://www.sciencedirect.com/science/article/pii/0014482783904433/pdf?md5=6f98198f2c1014b1747e5b0a338815a6&pid=1-s2.0-0014482783904433-main.pdf
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https://doi.org/10.1016/0014-4827(83)90443-3
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Citation

Faulstich, H., Trischmann, H., & Mayer, D. (1983). Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis. Experimental Cell Research, 144(1), 73-82. doi:10.1016/0014-4827(83)90443-3.


Cite as: https://hdl.handle.net/21.11116/0000-0003-68EF-C
Abstract
A fluorescent phallotoxin with high photostability, tetramethylrhodaminyl-phalloidin (Rh-phalloidin), has been prepared. The affinity of this compound to rabbit muscle actin has been determined to be about 6 times lower than that of phalloidin. In freshly isolated hepatocytes the internalized fluorescent toxin stains the cellular actin. In contrary, there is no actin staining visible in cultured hepatocytes. Short-term cultured hepatocytes (5 h of culturing) incorporate the toxin by endocytosis; it is kept sealed in the endocytotic vesicles, which are usually found accumulated at the sites where cells touch after reaggregation.