English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

A conformational transition of the sarcoplasmic reticulum calcium transport ATPase induced by vanadate

MPS-Authors
/persons/resource/persons93324

Hasselbach,  Wilhelm
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons232462

Medda,  Pankaj
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons94365

Migala,  Andrea
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons86998

Agostini,  Bruno
Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Hasselbach, W., Medda, P., Migala, A., & Agostini, B. (1983). A conformational transition of the sarcoplasmic reticulum calcium transport ATPase induced by vanadate. Zeitschrift für Naturforschung, C: Journal of Biosciences, 38(11-12), 1015-1022. doi:10.1515/znc-1983-11-1223.


Cite as: https://hdl.handle.net/21.11116/0000-0003-693E-3
Abstract
Vanadate binding to sarcoplasmic reticulum vesicles results in the loss of the externally located high affinity calcium binding sites of the calcium transport ATPase. Conversely the occupation by calcium of the internally located low affinity sites in the vanadate enzyme complex leads to the release of vanadate. Since the total number of calcium binding sites is not diminished by vanadate binding but slightly increases we conclude that vanadate binding induces a transition of the enzymes external high to internal low affinity calcium binding sites. The transposition of external to internal calcium binding sites is accompanied by a definite change in the structure of the sarcoplasmic reticulum membranes. On vanadate binding the asymmetrically arranged electron dense protein particles become symmetrically distributed.