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Journal Article

Two-photon Bessel beam tomography for fast volume imaging

MPS-Authors
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Seelig,  Johannes Dominik
Max Planck Research Group Neural Circuits, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Flores Valle,  Andres
Max Planck Research Group Neural Circuits, Center of Advanced European Studies and Research (caesar), Max Planck Society;

Fulltext (public)

2019_Seelig.pdf
(Publisher version), 23MB

Supplementary Material (public)
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Citation

Seelig, J. D., & Flores Valle, A. (2019). Two-photon Bessel beam tomography for fast volume imaging. Optics Express, 27(9), 12147-12162. doi:10.1364/OE.27.012147.


Cite as: http://hdl.handle.net/21.11116/0000-0003-876F-9
Abstract
Light microscopy on dynamic samples, for example neural activity in the brain, often requires imaging volumes that extend over several 100 µm in axial direction at a rate of at least several tens of Hertz. Here, we develop a tomography approach for scanning fluorescence microscopy which allows recording a volume image in a single frame scan. Volumes are imaged by simultaneously recording four independent projections at different angles using temporally multiplexed, tilted Bessel beams. From the resulting projections, three-dimensional images are reconstructed using inverse Radon transforms combined with convolutional neural networks (U-net).