Comparison of 2 Different Tumor Cell Labeling Techniques for in Vitro MRI 
Characterization with New Iron Oxide Particles

Saborowski, O; Santini, F; Fani, M; Robert, P; Ballet, S; Raynaud, JS; Santus, R; Froehlich, J; Scheffler, K; Bongartz, G; Maecke, HR

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Comparison of 2 Different Tumor Cell Labeling Techniques for in Vitro MRI Characterization with New Iron Oxide Particles

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https://cds.ismrm.org/ismrm-2008/files/1_program.pdf
(Abstract)

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Citation

Saborowski, O., Santini, F., Fani, M., Robert, P., Ballet, S., Raynaud, J., et al. (2008). Comparison of 2 Different Tumor Cell Labeling Techniques for in Vitro MRI Characterization with New Iron Oxide Particles. Poster presented at 16th Scientific Meeting and Exhibition of the International Society of Magnetic Resonance in Medicine (ISMRM 2008), Toronto, Canada.

Cite as: https://hdl.handle.net/21.11116/0000-0003-9330-0

Abstract

Iron oxide particles (SPIO and USPIO) are widely used for MRI-based cell labeling and tracking because they can be loaded on cells by simple protocols
and provide high image contrast due to their large susceptibility effect. We compared 2 different cell labeling models (AGAR and Ficoll) in human KB cells.
Both presented cell labeling methods (AGAR and Ficoll model) are feasible for in vitro MR characterization of KB tumor cells at 1.5, 2.35 and 3T. Ficoll
method is easier to perform because it does not need a heating procedure for preparation without air bubbles as compared to AGAR method.