English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

retroLEAP: rAAV-based retrograde trans-synaptic labeling, expression and perturbation

MPS-Authors
/persons/resource/persons238170

Sonntag,  Hannah
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons95439

Sprengel,  Rolf
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;
Olfaction Web, Max Planck Institute for Medical Research, Max Planck Society;
Rolf Sprengel Group, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
Citation

Reinert, J. K., Sonntag, I., Sonntag, H., Sprengel, R., Pelzer, P., Lessle, S., et al. (2019). retroLEAP: rAAV-based retrograde trans-synaptic labeling, expression and perturbation. bioRXiv, 1-13. doi:10.1101/537928.


Cite as: http://hdl.handle.net/21.11116/0000-0003-A0FF-9
Abstract
Identifying and manipulating synaptically connected neurons across brain regions remains a core challenge in understanding complex nervous systems. RetroLEAP is a novel approach for retrograde trans-synaptic Labelling, Expression And Perturbation. GFP-dependent recombinase (Flp-DOG) detects trans-synaptic transfer of GFP-tetanus toxin heavy chain fusion protein (GFP-TTC) and activates expression of any gene of interest in synaptically connected cells. RetroLEAP overcomes existing limitations, is non-toxic, highly flexible, efficient, sensitive and easy to implement.