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Journal Article

Dynamic and non-contact 3D sample rotation for microscopy.

MPS-Authors

Berndt,  Frederic
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Shah,  Gopi
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219546

Power,  Rory M
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons184379

Brugués,  Jan
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219250

Huisken,  Jan
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Berndt, F., Shah, G., Power, R. M., Brugués, J., & Huisken, J. (2018). Dynamic and non-contact 3D sample rotation for microscopy. Nature communications, 9(1): 5025. doi:10.1038/s41467-018-07504-3.


Cite as: https://hdl.handle.net/21.11116/0000-0003-F5A9-A
Abstract
Precise sample orientation is crucial for microscopy but is often performed with macroscopic tools and low accuracy. In vivo imaging of growing and developing samples even requires dynamic adaptation of the sample orientation to continuously achieve optimal imaging. Here, we present a method for freely positioning a sample in 3D by introducing magnetic beads and applying a magnetic field. We demonstrate magnetic orientation of fixed mouse embryos and artemia, and live zebrafish embryos and larvae on an epi-fluorescence microscope and on a light-sheet system for optimal imaging.