High-throughput single-cell transcriptomics on organoids.

Brazovskaja, Agnieska; Treutlein, Barbara; Camp, J Gray

doi:10.1016/j.copbio.2018.11.002

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High-throughput single-cell transcriptomics on organoids.

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Treutlein, Barbara
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Brazovskaja, A., Treutlein, B., & Camp, J. G. (2018). High-throughput single-cell transcriptomics on organoids. Current opinion in biotechnology, 55, 167-171. doi:10.1016/j.copbio.2018.11.002.

Cite as: https://hdl.handle.net/21.11116/0000-0003-F5B7-A

Abstract

Three-dimensional (3D) tissues grown in culture from human stem cells offer the incredible opportunity to analyze and manipulate human development, and to generate patient-specific models of disease. Methods to sequence DNA and RNA in single cells are being used to analyze these so-called 'organoid' systems in high-resolution. Single-cell transcriptomics has been used to quantitate the similarity of organoid cells to primary tissue counterparts in the brain, intestine, liver, and kidney, as well as identify cell-specific responses to environmental variables and disease conditions. The merging of these two technologies, single-cell genomics and organoids, will have profound impact on personalized medicine in the near future.