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Journal Article

Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians.

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Grohme,  Markus
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Vila-Farré,  Miquel
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Rink,  Jochen
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Grohme, M., Vila-Farré, M., & Rink, J. (2018). Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians. Methods in molecular biology (Clifton, N.J.), 1774, 267-275. doi:10.1007/978-1-4939-7802-1_7.


Cite as: https://hdl.handle.net/21.11116/0000-0003-F5F1-8
Abstract
High-quality genomic DNA extraction is a starting point for many downstream applications in modern molecular biology. Here, we describe a simple method for isolating high molecular weight genomic DNA from planarians. The method is based on tissue lysis by a mixture of a chaotropic salt and detergent followed by organic extraction to remove proteins and lipids followed by a postpurification step to remove contaminating polysaccharides. The isolated DNA is of high molecular weight and compatible with polymerase chain reaction, cloning, or next-generation sequencing library preparation.