Total RNA Isolation from Planarian Tissues.

Liu, Shang-Yun; Rink, Jochen

doi:10.1007/978-1-4939-7802-1_6

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Total RNA Isolation from Planarian Tissues.

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Liu, Shang-Yun
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Rink, Jochen
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Liu, S.-Y., & Rink, J. (2018). Total RNA Isolation from Planarian Tissues. Methods in molecular biology (Clifton, N.J.), 1774, 259-265. doi:10.1007/978-1-4939-7802-1_6.

Cite as: https://hdl.handle.net/21.11116/0000-0003-F630-1

Abstract

Isolation of high-quality and nondegraded RNA is a prerequisite for many modern molecular biology applications. A variety of RNA extraction products and protocols are available for the standard model organisms, yet species-specific peculiarities of less well studied organisms often require specific protocol adaptations. Here we describe a robust RNA extraction protocol for planarians that is widely used in the community. The protocol combines tissue homogenization in TRIzol with phenol-chloroform extraction and subsequent purification over commercial columns. The purified RNA can be used for many downstream applications, such as cDNA synthesis and next-generation sequencing (NGS, RNA-seq).