The RNA-binding protein PTBP1 is necessary for B cell selection in germinal 
centers.

Monzón-Casanova, Elisa; Screen, Michael; Díaz-Muñoz, Manuel D; Coulson, Richard M R; Bell, Sarah E; Lamers, Greta; Solimena, Michele; Smith, Christopher W J; Turner, Martin

doi:10.1038/s41590-017-0035-5

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The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers.

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Solimena, Michele
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Monzón-Casanova, E., Screen, M., Díaz-Muñoz, M. D., Coulson, R. M. R., Bell, S. E., Lamers, G., et al. (2018). The RNA-binding protein PTBP1 is necessary for B cell selection in germinal centers. Nature immunology, 19(3), 267-278. doi:10.1038/s41590-017-0035-5.

Cite as: https://hdl.handle.net/21.11116/0000-0003-F64F-0

Abstract

Antibody affinity maturation occurs in germinal centers (GCs), where B cells cycle between the light zone (LZ) and the dark zone. In the LZ, GC B cells bearing immunoglobulins with the highest affinity for antigen receive positive selection signals from helper T cells, which promotes their rapid proliferation. Here we found that the RNA-binding protein PTBP1 was needed for the progression of GC B cells through late S phase of the cell cycle and for affinity maturation. PTBP1 was required for proper expression of the c-MYC-dependent gene program induced in GC B cells receiving T cell help and directly regulated the alternative splicing and abundance of transcripts that are increased during positive selection to promote proliferation.