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Journal Article

An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana

MPS-Authors
/persons/resource/persons273386

Wu,  R
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273608

Lucke,  M
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273613

Jang,  Y-T
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons272318

Zhu,  W
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons272956

Symeonidi,  E
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons273615

Wang,  C
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons272720

Fitz,  J
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons272723

Wang,  X
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons271416

Schwab,  R
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons85266

Weigel,  D
Department Molecular Biology, Max Planck Institute for Developmental Biology, Max Planck Society;

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Citation

Wu, R., Lucke, M., Jang, Y.-T., Zhu, W., Symeonidi, E., Wang, C., et al. (2018). An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana. Plant Methods, 14: 65. doi:10.1186/s13007-018-0330-7.


Cite as: https://hdl.handle.net/21.11116/0000-0003-B674-D
Abstract
Background: Our knowledge of natural genetic variation is increasing at an extremely rapid pace, affording an opportunity to come to a much richer understanding of how effects of specific genes are dependent on the genetic background. To achieve a systematic understanding of such GxG interactions, it is desirable to develop genome editing tools that can be rapidly deployed across many different genetic varieties. Results: We present an efficient CRISPR/Cas9 toolbox of super module (SM) vectors. These vectors are based on a previously described fluorescence protein marker expressed in seeds allowing identification of transgene-free mutants. We have used this vector series to delete genomic regions ranging from 1.7 to 13 kb in different natural accessions of the wild plant Arabidopsis thaliana. Based on results from 53 pairs of sgRNAs targeting individual nucleotide binding site leucine-rich repeat (NLR) genes, we provide a comprehensive overview of obtaining heritable deletions. Conclusions: The SM series of CRISPR/Cas9 vectors enables the rapid generation of transgene-free, genome edited plants for a diversity of functional studies.