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Bestimmung metabolischer Profile des Weizenpathogens Zymoseptoria tritici und seiner Wirtspflanze Weizen mittels FT-ICR-MS

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Citation

Reim, E. (2018). Bestimmung metabolischer Profile des Weizenpathogens Zymoseptoria tritici und seiner Wirtspflanze Weizen mittels FT-ICR-MS. Master Thesis, Christian-Albrechts-Universität, Kiel.


Abstract
Septoria tritici blotch (STB), one of the most distributed diseases referring to wheat (Triticum aestivum) is caused by Zymoseptoria tritici. This pathogen is characterized by a biphasic infection phase: the long asymptomatic phase and the necrotrophic phase.
The present thesis investigates the metabolic profiles of wheat and its pathogen Z. tritici by means of FT-ICR-MS. The aim of this thesis was to identify the metabolites which are released during the infection phases. The focus was on wheat metabolites, metabolites of the resistant and susceptible wheat cultivars (Chinese Spring, Obelisk), and fungus metabolites.
The results of the study show that the metabolic profiles of the susceptible variety Obelisk and the resistant variety Chinese Spring differ after the infection with Z. tritici. In this work, the metabolic profiles of the plant-pathogen interaction in both wheat varieties were partially elucidated. Fungal metabolites, such as mannitol, arabitol and stachydrine, were identified in the susceptible wheat variety. The resistance of the Chinese Spring variety was related to DIMBOA.