124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics

Wade, Orsolya K.; Woehrstein, Johannes B.; Nickels, Philipp C.; Strauss, Sebastian; Stehr, Florian; Stein, Johannes; Schueder, Florian; Strauss, Maximilian T.; Ganji, Mahipal; Schnitzbauer, Joerg; Grabmayr, Heinrich; Yin, Peng; Schwille, Petra; Jungmann, Ralf

doi:10.1021/acs.nanolett.9b00508

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124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics

MPG-Autoren

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Wade, Orsolya K.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Woehrstein, Johannes B.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons224551

Nickels, Philipp C.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons208765

Strauss, Sebastian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229551

Stehr, Florian
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Stein, Johannes
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

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Schueder, Florian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons186093

Strauss, Maximilian T.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229346

Ganji, Mahipal
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons208763

Schnitzbauer, Joerg
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons215437

Grabmayr, Heinrich
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons15815

Schwille, Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons172959

Jungmann, Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Zitation

Wade, O. K., Woehrstein, J. B., Nickels, P. C., Strauss, S., Stehr, F., Stein, J., et al. (2019). 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics. Nano Letters, 19(4), 2641-2646. doi:10.1021/acs.nanolett.9b00508.

Zitierlink: https://hdl.handle.net/21.11116/0000-0003-DD6E-A

Zusammenfassung

Optical super-resolution techniques reach unprecedented spatial resolution down to a few nanometers. However, efficient multiplexing strategies for the simultaneous detection of hundreds of molecular species are still elusive. Here, we introduce an entirely new approach to multiplexed super-resolution microscopy by designing the blinking behavior of targets with engineered binding frequency and duration in DNA-PAINT. We assay this kinetic barcoding approach in silico and in vitro using DNA origami structures, show the applicability for multiplexed RNA and protein detection in cells, and finally experimentally demonstrate 124-plex super-resolution imaging within minutes.