MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording

Chen, Y; Pais-Roldán, P; Chen, X; Frosz, MH; Yu, X

doi:10.1038/s41467-019-10450-3

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MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording

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Chen, Y
Research Group Translational Neuroimaging and Neural Control, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Pais-Roldán, P
Research Group Translational Neuroimaging and Neural Control, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Chen, X
Research Group Translational Neuroimaging and Neural Control, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Yu, X
Research Group Translational Neuroimaging and Neural Control, Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Citation

Chen, Y., Pais-Roldán, P., Chen, X., Frosz, M., & Yu, X. (2019). MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca2+ recording. Nature Communications, 10(1): 2536. doi:10.1038/s41467-019-10450-3.

Cite as: https://hdl.handle.net/21.11116/0000-0003-C3E2-1

Abstract

Optical fiber-mediated optogenetic activation and neuronal Ca2+ recording in combination with fMRI provide a multi-modal fMRI platform. Here, we developed an MRI-guided robotic arm (MgRA) as a flexible positioning system with high precision to real-time assist optical fiber brain intervention for multi-modal animal fMRI. Besides the ex vivo precision evaluation, we present the highly reliable brain activity patterns in the projected basal forebrain regions upon MgRA-driven optogenetic stimulation in the lateral hypothalamus. Also, we show the step-wise optical fiber targeting thalamic nuclei and map the region-specific functional connectivity with whole-brain fMRI accompanied by simultaneous calcium recordings to specify its circuit-specificity. The MgRA also guides the real-time microinjection to specific deep brain nuclei, which is demonstrated by an Mn-enhanced MRI method. The MgRA represents a clear advantage over the standard stereotaxic-based fiber implantation and opens a broad avenue to investigate the circuit-specific functional brain mapping with the multi-modal fMRI platform.