Identification of Potential Novel Interacting Partners for Coagulation Factor 
XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder

Singh, Sneha; Akhter, Mohammad Suhail; Dodt, Johannes; Volkers, Peter; Reuter, Andreas; Reinhart, Christoph; Krettler, Christoph; Oldenburg, Johannes; Biswas, Arijit

doi:10.3390/ijms20112682

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Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder

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Reinhart, Christoph
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Krettler, Christoph
Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society;

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引用

Singh, S., Akhter, M. S., Dodt, J., Volkers, P., Reuter, A., Reinhart, C., Krettler, C., Oldenburg, J., & Biswas, A. (2019). Identification of Potential Novel Interacting Partners for Coagulation Factor XIII B (FXIII-B) Subunit, a Protein Associated with a Rare Bleeding Disorder. International Journal of Molecular Sciences, 20(11):. doi:10.3390/ijms20112682.

引用: https://hdl.handle.net/21.11116/0000-0003-C880-A

要旨

Coagulation factor XIII (FXIII) is a plasma-circulating heterotetrameric pro-transglutaminase complex that is composed of two catalytic FXIII-A and two protective/regulatory FXIII-B subunits. FXIII acts by forming covalent cross-links within a preformed fibrin clots to prevent its premature fibrinolysis. The FXIII-A subunit is known to have pleiotropic roles outside coagulation, but the FXIII-B subunit is a relatively unexplored entity, both structurally as well as functionally. Its discovered roles so far are limited to that of the carrier/regulatory protein of its partner FXIII-A subunit. In the present study, we have explored the co-presence of protein excipients in commercial FXIII plasma concentrate FibrogamminP by combination of protein purification and mass spectrometry-based verification. Complement factor H was one of the co-excipients observed in this analysis. This was followed by performing pull down assays from plasma in order to detect the putative novel interacting partners for the FXIII-B subunit. Complement system proteins, like complement C3 and complement C1q, were amongst the proteins that were pulled down. The only protein that was observed in both experimental set ups was alpha-2-macroglobulin, which might therefore be a putative interacting partner of the FXIII/FXIII-B subunit. Future functional investigations will be needed to understand the physiological significance of this association.