Tissue-specific profiling of membrane proteins in the salicin sequestering 
juveniles of the herbivorous leaf beetle, Chrysomela populi

Schmidt, Lydia; Wielsch, Natalie; Wang, Ding; Boland, Wilhelm; Burse, Antje

doi:10.1016/j.ibmb.2019.03.009

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Tissue-specific profiling of membrane proteins in the salicin sequestering juveniles of the herbivorous leaf beetle, Chrysomela populi

MPS-Authors

/persons/resource/persons185801

Schmidt, Lydia
Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons16217

Wielsch, Natalie
Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons130287

Wang, Ding
Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;
IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons3812

Boland, Wilhelm
Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons3825

Burse, Antje
Research Group Dr. A. Burse, Chemical Defense of Leaf Beetles, Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Schmidt, L., Wielsch, N., Wang, D., Boland, W., & Burse, A. (2019). Tissue-specific profiling of membrane proteins in the salicin sequestering juveniles of the herbivorous leaf beetle, Chrysomela populi. Insect Biochemistry and Molecular Biology, 109, 81-91. doi:10.1016/j.ibmb.2019.03.009.

引用: https://hdl.handle.net/21.11116/0000-0003-D270-1

要旨

Sequestration of plant secondary metabolites is a detoxification strategy widespread in herbivorous insects including
not only storage, but also usage of these metabolites for the insects' own benefit. Larvae of the poplar leaf
beetle Chrysomela populi sequester plant-derived salicin to produce the deterrent salicylaldehyde in specialized
exocrine glands. To identify putative transporters involved in the sequestration process we investigated integral
membrane proteins of several tissues from juvenile C. populi by using a proteomics approach. Computational
analyses led to the identification of 122 transport proteins in the gut, 105 in the Malpighian tubules, 94 in the fat
body and 27 in the defensive glands. Among these, primary active transporters as well as electrochemical potential-
driven transporters were most abundant in all tissues, including ABC transporters (especially subfamilies
B, C and G) and sugar porters as most interesting families facilitating the sequestration of plant glycosides.
Whereas ABC transporters are predominantly expressed simultaneously in several tissues, sugar porters are often
expressed in only one tissue, suggesting that sugar porters govern more distinct functions than members of the
ABC family. The inventory of transporters presented in this study provides the base for further functional
characterizations on transport processes of sequestered glycosides in insects.