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A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes

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Steyer,  Anna M.
Electron microscopy, Neurogenetics, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Möbius,  Wiebke
Electron microscopy, Neurogenetics, Max Planck Institute of Experimental Medicine, Max Planck Society;

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Citation

So, C., Seres, K. B., Steyer, A. M., Mönnich, E., Clift, D., Pejkovska, A., et al. (2019). A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science, 364(6447): eaat9557. doi:10.1126/science.aat9557.


Cite as: https://hdl.handle.net/21.11116/0000-0003-FC97-7
Abstract
Chromosome segregation typically requires centrosomes, which generate the microtubule spindle. However, mammalian eggs build a spindle and segregate chromosomes without centrosomes. How acentrosomal spindles are organized has remained elusive. So et al. show that centrosomal and microtubule-associated proteins are repurposed into a large “liquid-like meiotic spindle domain” (LISD) in eggs. The domains localized to spindle poles and also extended to the spindle fibers that connect to kinetochores. LISDs formed by phase separation and were required for spindle assembly, serving as reservoirs that locally sequester and mobilize spindle assembly factors within the large egg cytoplasm.