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Journal Article

Absolute quantification of cohesin, CTCF and their regulators in human cells.

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Politi,  A.
Research Group of Cytoskeletal Dynamics in Oocytes, MPI for Biophysical Chemistry, Max Planck Society;

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3134900.pdf
(Publisher version), 9MB

Supplementary Material (public)

3134900_Suppl_1.htm
(Supplementary material), 17KB

3134900_Suppl_2.htm
(Supplementary material), 15KB

3134900_Suppl_3.pdf
(Supplementary material), 321KB

Citation

Holzmann, J., Politi, A., Nagasaka, K., Hantsche-Grininger, M., Walther, N., Koch, B., et al. (2019). Absolute quantification of cohesin, CTCF and their regulators in human cells. eLife, 8: e46269. doi:10.7554/eLife.46269.


Cite as: http://hdl.handle.net/21.11116/0000-0004-3ED0-C
Abstract
The organisation of mammalian genomes into loops and topologically associating domains (TADs) contributes to chromatin structure, gene expression and recombination. TADs and many loops are formed by cohesin and positioned by CTCF. In proliferating cells, cohesin also mediates sister chromatid cohesion, which is essential for chromosome segregation. Current models of chromatin folding and cohesion are based on assumptions of how many cohesin and CTCF molecules organise the genome. Here we have measured absolute copy numbers and dynamics of cohesin, CTCF, NIPBL, WAPL and sororin by mass spectrometry, fluorescence-correlation spectroscopy and fluorescence recovery after photobleaching in HeLa cells. In G1-phase, there are similar to 250,000 nuclear cohesin complexes, of which similar to 160,000 are chromatin-bound. Comparison with chromatin immunoprecipitation-sequencing data implies that some genomic cohesin and CTCF enrichment sites are unoccupied in single cells at any one time. We discuss the implications of these findings for how cohesin can contribute to genome organisation and cohesion.