English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Quantification of blumenol derivatives as leaf biomarkers for plant-AMF association

MPS-Authors
/persons/resource/persons239917

Mindt,  Elisabeth
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons199274

Wang,  Ming
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;
IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons27714

Schäfer,  Martin
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons3907

Halitschke,  Rayko
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;

/persons/resource/persons3786

Baldwin,  Ian Thomas
Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society;

External Resource
Fulltext (public)

ITB611.pdf
(Publisher version), 210KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Mindt, E., Wang, M., Schäfer, M., Halitschke, R., & Baldwin, I. T. (2019). Quantification of blumenol derivatives as leaf biomarkers for plant-AMF association. Bio-protocol, 9(14): e3301. doi:10.21769/BioProtoc.3301.


Cite as: http://hdl.handle.net/21.11116/0000-0004-520B-4
Abstract
Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, efficient characterization methods are required to monitor and quantify plant-AMF colonization. Existing methods, based on destructive sampling and elaborate root tissue analysis, are of limited value for high-throughput (HTP) screening. Here we describe a detailed protocol for the HTP quantification of blumenol derivatives in leaves by a simple extraction procedure and sensitive liquid chromatography mass spectrometry (LC/MS) analysis as accurate proxies of root AMF-associations in both model plants and economically relevant crops.