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External RBS/PIXE analysis for evaluating quantum dots internalization into HeLa cells.

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Koike,  S.
Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Saito, M., Koike, S., Holm, K., Bregolin, F. L., & Hofsäss, H. (2019). External RBS/PIXE analysis for evaluating quantum dots internalization into HeLa cells. Nuclear Instruments and Methods in Physics Research B, 450, 173-178. doi:10.1016/j.nimb.2018.08.027.


Cite as: http://hdl.handle.net/21.11116/0000-0004-5237-2
Abstract
We have developed an external RBS/PIXE setup at University of Gottingen. By using this setup, the analysis can be performed in atmospheric condition, which leads to realizing a quantitative analysis for volatile samples with depth information. We applied this technique for HeLa cells, internalized CdSe quantum dots (Q-dot). External RBS shows the depth difference of Q-dot depth distribution in cells, w or w/o the treatment with an endocytosis inhibitor, which agrees well with the result of confocal microscopy. The quantity of Q-dot is also evaluated by external PIXE, which coincides with the result of RBS and fluorescence measurement. In addition, the application of Si3N4 film as a medium for HeLa cells is presented.