Cyclic nucleotide-specific optogenetics highlights compartmentalization of the 
sperm flagellum into cAMP microdomains.

Raju, Diana N.; Hansen, Jan N.; Rassmann, Sebastian; Stüven, Birthe; Jikeli, Jan F.; Strünker, Timo; Körschen, Heinz Gerd; Möglich, Andreas; Wachten, Dagmar

doi:10.3390/cells8070648

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Cyclic nucleotide-specific optogenetics highlights compartmentalization of the sperm flagellum into cAMP microdomains.

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Körschen, Heinz Gerd
Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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Wachten, Dagmar
Max Planck Research Group Molecular Physiology, Center of Advanced European Studies and Research (caesar), Max Planck Society;

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2019_RajuKörschenWachten_CyclicNucleotide-Specific Optogenetics_Cells.pdf
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Citation

Raju, D. N., Hansen, J. N., Rassmann, S., Stüven, B., Jikeli, J. F., Strünker, T., et al. (2019). Cyclic nucleotide-specific optogenetics highlights compartmentalization of the sperm flagellum into cAMP microdomains. Cells, 8(7): 648. doi:10.3390/cells8070648.

Cite as: https://hdl.handle.net/21.11116/0000-0004-69F2-5

Abstract

Inside the female genital tract, mammalian sperm undergo a maturation process called capacitation, which primes the sperm to navigate across the oviduct and fertilize the egg. Sperm capacitation and motility are controlled by 3',5'-cyclic adenosine monophosphate (cAMP). Here, we show that optogenetics, the control of cellular signaling by genetically encoded light-activated proteins, allows to manipulate cAMP dynamics in sperm flagella and, thereby, sperm capacitation and motility by light. To this end, we used sperm that express the light-activated phosphodiesterase LAPD or the photo-activated adenylate cyclase bPAC. The control of cAMP by LAPD or bPAC combined with pharmacological interventions provides spatiotemporal precision and allows to probe the physiological function of cAMP compartmentalization in mammalian sperm.