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Journal Article

Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation

MPS-Authors

Collmann,  F. M.
Max Planck Institute for Biology of Ageing, Max Planck Society;

Pijnenburg,  R.
Max Planck Institute for Biology of Ageing, Max Planck Society;

Schneider,  G.
Max Planck Institute for Biology of Ageing, Max Planck Society;

Schafer,  C.
Max Planck Institute for Biology of Ageing, Max Planck Society;

Folz-Donahue,  K.
Max Planck Institute for Biology of Ageing, Max Planck Society;

Kukat,  C.
Max Planck Institute for Biology of Ageing, Max Planck Society;

Hoehn,  M.
Max Planck Institute for Biology of Ageing, Max Planck Society;

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Citation

Collmann, F. M., Pijnenburg, R., Schneider, G., Schafer, C., Folz-Donahue, K., Kukat, C., et al. (2018). Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation. J Neuroimmune Pharmacol, 13(3), 371-382. doi:10.1007/s11481-018-9789-2.


Cite as: https://hdl.handle.net/21.11116/0000-0004-7201-A
Abstract
Microglial cells as innate immune key players have a critical and unique role in neurodegenerative disorders. They strongly interact with their microenvironment in a complex manner and react to changes by switching their phenotype and functional activation states. In order to understand the development of brain diseases, it is imperative to elucidate up- or down-regulation of genes involved in microglia polarisation in time-profile by a simple-to-use strategy. Here, we present a new imaging strategy to follow promoter activity of genes involved in microglia polarisation. We lentivirally transduced BV-2 microglia cells in culture with constructs consisting of the induced nitric oxide synthase (iNOS), Fc gamma receptor III (Fcgr3) (both resembling the pro-inflammatory M1-like phenotype) or Chitinase-like 3 (Chil3/Ym1) (resembling the anti-inflammatory M2-like phenotype) promoters and stimulated transgenic cells with potent activators for pro- or anti-inflammatory response, such as lipopolysaccharide (LPS) + interferon gamma (IFN-gamma) or interleukin (IL)-4, respectively. Promoter activities upon polarisation phases were quantitatively assessed by the two imaging reporters Luc2 for bioluminescence and eGFP for fluorescence.