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Anaerobic mineralization of quaternary carbon atoms: Isolation of denitrifying bacteria on dimethylmalonate

MPS-Authors
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Kniemeyer,  Olaf
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Probian,  Christina
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Rosselló-Mora,  Ramon
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Harder,  Jens
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Kniemeyer, O., Probian, C., Rosselló-Mora, R., & Harder, J. (1999). Anaerobic mineralization of quaternary carbon atoms: Isolation of denitrifying bacteria on dimethylmalonate. Applied and Environmental Microbiology, 65(8), 3319-3324.


Cite as: https://hdl.handle.net/21.11116/0000-0005-4833-1
Abstract
The microbial capacity to degrade simple organic compounds with quaternary carbon atoms was demonstrated by enrichment and isolation of five denitrifying strains on dimethylmalonate as the sole electron donor and carbon source. Quantitative growth experiments showed a complete mineralization of dimethylmalonate. According to phylogenetic analysis of the complete 16S rRNA genes, two strains isolated from activated sewage sludge were related to the genus Paracoccus within the alpha-Proteobacteria (98.0 and 98.2% 16S rRNA gene similarity to Paracoccus dentrificans(T)), and three strains isolated from freshwater ditches were affiliated with the P-Proteobacteria (97.4 and 98.3% 16S rRNA gene similarity to Herbaspirillum seropedicae(T) and Acidovorax facilis(T), respectively). Most-probable-number determinations for denitrifying populations in sewage sludge yielded 4.6 x 10(4) dimethylmalonate utilizing cells ml(-1), representing up to 0.4% of the total culturable nitrate-reducing population.