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Anaerobic oxidation of o-xylene, m-xylene, and homologous alkylbenzenes by new types of sulfate-reducing bacteria

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Zengler,  Karsten
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Rabus,  Ralf
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

Aeckersberg,  F.
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Rosselló-Mora,  Ramon
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Widdel,  Friedrich
Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Zengler_1999.pdf
(Publisher version), 612KB

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Citation

Harms, G., Zengler, K., Rabus, R., Aeckersberg, F., Minz, D., Rosselló-Mora, R., et al. (1999). Anaerobic oxidation of o-xylene, m-xylene, and homologous alkylbenzenes by new types of sulfate-reducing bacteria. Applied and Environmental Microbiology, 65(3), 999-1004.


Cite as: http://hdl.handle.net/21.11116/0000-0005-4E88-B
Abstract
Various alkylbenzenes were depleted during growth of an anaerobic, sulfate-reducing enrichment culture with crude oil as the only source of organic substrates. From this culture, two new types of mesophilic, rod-shaped sulfate-reducing bacteria, strains oXyS1 and mXyS1, were isolated with o-xylene and m-xylene, respectively, as organic substrates. Sequence analyses of 16S rRNA genes revealed that the isolates affiliated with known completely oxidizing sulfate-reducing bacteria of the delta subclass of the class Proteobacteria. Strain oXyS1 showed the highest similarities to Desulfobacterium cetonicum and Desulfosarcina variabilis (similarity values, 98.4 and 98.7%, respectively). Strain mXyS1 was less closely related to known species, the closest relative being Desulfococcus multivorans (similarity value, 86.9%). Complete mineralization of o-xylene and m-xylene was demonstrated in quantitative growth experiments. Strain oXyS1 was able to utilize toluene, o-ethyltoluene, benzoate, and o-methylbenzoate in addition to o-xylene. Strain mXyS1 oxidized toluene, m-ethyltoluene, m-isoproyltoluene, benzoate, and m-methylbenzoate in addition to m-xylene. Strain oXyS1 did not utilize m-alkyltoluenes, whereas strain mXyS1 did not utilize o-alkyltoluenes. Like the enrichment culture, both isolates grew anaerobically on crude oil with concomitant reduction of sulfate to sulfide.