In situ detection of Escherichia coli cells containing ColE1-related plasmids 
by hybridization to regulatory RNA II

Juretschko, S.; Schönhuber, Wilhelm; Kulakauskas, S.; Ehrlich, D.S.; Schleifer, K.H.; Amann, Rudolf I.

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In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II

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Schönhuber, Wilhelm
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Amann, Rudolf I.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Juretschko, S., Schönhuber, W., Kulakauskas, S., Ehrlich, D., Schleifer, K., & Amann, R. I. (1999). In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II. Systematic and Applied Microbiology, 22(1), 1-8.

Cite as: https://hdl.handle.net/21.11116/0000-0005-47BA-A

Abstract

A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regulatory RNA I did not result in the in situ detection of plasmid-bearing cells.