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A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples

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Holst,  Gerhard A.
Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Kohls,  Oliver
Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Klimant,  Ingo
Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society;

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König,  Bettina
Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Kühl,  Michael
Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Holst, G. A., Kohls, O., Klimant, I., König, B., Kühl, M., & Richter, I. (1998). A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples. Sensors and Actuators b-Chemical, 51(1-3), 163-170. doi:10.1016/S0925-4005(98)00232-9.


Cite as: http://hdl.handle.net/21.11116/0000-0005-432F-C
Abstract
We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 mu s to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources, A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed, (C) 1998 Elsevier Science S.A. All rights reserved.