Application of 23S rDNA-targeted oligonucleotide probes specific for 
enterococci to water hygiene control

Frahm, E.; Heiber, I.; Hoffmann, S.; Koob, C.; Meier, H.; Ludwig, W.; Amann, Rudolf I.; Schleifer, K.H.; Obst, U.

doi:10.1016/S0723-2020(98)80055-6

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Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control

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Amann, Rudolf I.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Frahm, E., Heiber, I., Hoffmann, S., Koob, C., Meier, H., Ludwig, W., et al. (1998). Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control. Systematic and Applied Microbiology, 21(3), 450-453. doi:10.1016/S0723-2020(98)80055-6.

Cite as: https://hdl.handle.net/21.11116/0000-0005-42A1-A

Abstract

Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.