Improved sensitivity of whole-cell hybridization by the combination of 
horseradish peroxidase-labeled oligonucleotides and tyramide signal 
amplification

Schönhuber, Wilhelm; Fuchs, Bernhard M.; Juretschko, S; Amann, Rudolf I.

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification

MPS-Authors

/persons/resource/persons210759

Schönhuber, Wilhelm
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

/persons/resource/persons210389

Fuchs, Bernhard M.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

/persons/resource/persons210230

Amann, Rudolf I.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

Schoenhuber_1997.pdf
(出版社版), 2MB

付随資料 (公開)

There is no public supplementary material available

引用

Schönhuber, W., Fuchs, B. M., Juretschko, S., & Amann, R. I. (1997). Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Applied and Environmental Microbiology, 63(8), 3268-3273.

引用: https://hdl.handle.net/21.11116/0000-0005-0AFA-7

要旨

The substrate fluorescein-tyramide was combined with oligonucleotide probes directly labeled with horseradish peroxidase to improve the sensitivity of in situ hybridization of whole fixed bacterial cells. Flow cytometry and quantitative microscopy of cells hybridized by this technique showed 10- to 20-fold signal amplifications relative to fluorescein-manolabeled probes. The application of the new technique to the detection of natural bacterial communities resulted in very bright signals; however, the number of detected cells was significantly lower than that detected with fluorescently monolabeled, rRNA-targeted oligonucleotide probes.