Improved sensitivity of whole-cell hybridization by the combination of 
horseradish peroxidase-labeled oligonucleotides and tyramide signal 
amplification

Schönhuber, Wilhelm; Fuchs, Bernhard M.; Juretschko, S; Amann, Rudolf I.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification

MPS-Authors

/persons/resource/persons210759

Schönhuber, Wilhelm
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

/persons/resource/persons210389

Fuchs, Bernhard M.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

/persons/resource/persons210230

Amann, Rudolf I.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

Schoenhuber_1997.pdf
(Publisher version), 2MB

Supplementary Material (public)

There is no public supplementary material available

Citation

Schönhuber, W., Fuchs, B. M., Juretschko, S., & Amann, R. I. (1997). Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Applied and Environmental Microbiology, 63(8), 3268-3273.

Cite as: https://hdl.handle.net/21.11116/0000-0005-0AFA-7

Abstract

The substrate fluorescein-tyramide was combined with oligonucleotide probes directly labeled with horseradish peroxidase to improve the sensitivity of in situ hybridization of whole fixed bacterial cells. Flow cytometry and quantitative microscopy of cells hybridized by this technique showed 10- to 20-fold signal amplifications relative to fluorescein-manolabeled probes. The application of the new technique to the detection of natural bacterial communities resulted in very bright signals; however, the number of detected cells was significantly lower than that detected with fluorescently monolabeled, rRNA-targeted oligonucleotide probes.