English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Use of green fluorescent protein as a marker for ecological studies of activated sludge communities

MPS-Authors
/persons/resource/persons210230

Amann,  Rudolf I.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Eberl, L., Schulze, R., Ammendola, A., Geisenberger, O., Erhart, R., Sternberg, C., et al. (1997). Use of green fluorescent protein as a marker for ecological studies of activated sludge communities. FEMS Microbiology Letters, 149(1), 77-83. doi:10.1016/S0378-1097(97)00058-X.


Cite as: http://hdl.handle.net/21.11116/0000-0005-0A76-C
Abstract
The potential of the green fluorescent protein (GFP) as a marker gene for ecological investigations of an activated sludge community was assessed. By inserting the hybrid transposon mini-Tn5 gfp into the chromosome of Pseudomonas putida KT2442 a strongly fluorescent mutant was obtained. This strain was used for in vivo tracking of individual cells after introduction into a simple sludge microcosm. It is demonstrated that the observed reduction of introduced bacteria from sewage is mainly the result of predation by protozoa. The feasibility of combining detection of GFP fluorescence with whole cell hybridization employing fluorescently labeled, rRNA-targeted oligonucleotides in paraformaldehyde fixed samples is demonstrated.