English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Other

Identification of sulfate-reducing bacteria using 16S rRNA binding oligonucleotide probes

MPS-Authors

Ramsing,  NB
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

/persons/resource/persons210554

Kühl,  Michael
Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society;

/persons/resource/persons210489

Jørgensen,  Bo Barker
Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Abstract
Fluorescent-dye-conjugated oligonucleotides were used as "phylogenetic" probes to identify sulfate reducing bacteria (SRB) in a photosynthetic biofilm. Two samples of biofilm from a trickling filter in a waste-water treatment plant were incubated for 48 hr's; one in darkness, the other in daylight. Chemical gradients of (02, S2-, and pH) in the biofiIn1s were then measured with microelectrodes prior to rapid freezing and slicing on a cryo-microtome. The vertical slices were stained with three different probes: a sulfate reducing bacterial (inclusive for most species of the 8-group of purple bacteria), a Desulfobacter, and a non-hybridising control probe. A good correlation between the vertical distribution of SRB and the measured 02-profile was found, with the SRB being restricted to the deeper anaerobic layers.