Identification of sulphate-reducing ectosymbiotic bacteria from anaerobic 
ciliates using 16S rRNA binding oligonucleotide probes

Fenchel, T.; Ramsing, N.B.

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Identification of sulphate-reducing ectosymbiotic bacteria from anaerobic ciliates using 16S rRNA binding oligonucleotide probes

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Ramsing, N.B.
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Citation

Fenchel, T., & Ramsing, N. (1992). Identification of sulphate-reducing ectosymbiotic bacteria from anaerobic ciliates using 16S rRNA binding oligonucleotide probes. Archives of Microbiology, 158(6), 394-397.

Cite as: https://hdl.handle.net/21.11116/0000-0004-82F8-1

Abstract

The identity of ectosymbiotic bacteria of some marine, free-living anaerobic ciliates (Metopus contortus, Caenomorpha levanderi and Parablepharisma sp.) was studied using fluorescent-dye-conjugated oligonucleotides complementary to short sequence elements of 16S ribosomal RNA. The ectosymbiotic bacteria of all species hybridized with a eubacterial probe and those of the two former mentioned species hybridized with a general probe for sulphate-reducing bacteria, but not to a probe specific for Desulfobacter. The results support indirect evidence suggesting that ectosymbiotic bacteria of anaerobic ciliates are sulphate-reducers which depend on host metabolites for substrates.