English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging

MPS-Authors
There are no MPG-Authors in the publication available
External Resource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Mueller, P., Schuermann, M., Girardo, S., Cojoc, G., & Guck, J. (2018). Accurate evaluation of size and refractive index for spherical objects in quantitative phase imaging. OPTICS EXPRESS, 26(8), 10729-10743. doi:10.1364/OE.26.010729.


Cite as: http://hdl.handle.net/21.11116/0000-0004-AF48-7
Abstract
Measuring the average refractive index (RI) of spherical objects, such as suspended cells, in quantitative phase imaging (QPI) requires a decoupling of RI and size from the QPI data. This has been commonly, achieved by determining the object's radius with geometrical approaches, neglecting light-scattering. Here, we present a novel QPI Fitting algorithm that reliably uncouples the RI using Mie theory and a semi-analytical, corrected Rytov approach. We assess the range of validity of this algorithm in silico and experimentally investigate various objects (oil and protein droplets, microgel beads, cells) and noise conditions. In addition, we provide important practical cues for the analysis of spherical objects in QPI. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.