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Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells

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Herbig, M., Kraeter, M., Plak, K., Mueller, P., Guck, J., & Otto, O. (2018). Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells. In FLOW CYTOMETRY PROTOCOLS, 4TH EDITION (pp. 347-369). 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA: HUMANA PRESS INC.

Cite as: https://hdl.handle.net/21.11116/0000-0004-AF29-A
Real-time deformability cytometry (RT-DC) is a microfluidic technique that allows to capture and evaluate morphology and rheology of up to 1000 cells/s in a constricted channel. The cells are deformed without mechanical contact by hydrodynamic forces and are quantified in real-time without the need of additional handling or staining procedures. Segmented pictures of the cells are stored and can be used for further analysis. RT-DC is sensitive to alterations of the cytoskeleton, which allows, e.g., to show differences in cell cycle phases, identify different subpopulations in whole blood and to study mechanical stiffening of cells entering a dormant state. The abundance of the obtainable parameters and the interpretation as mechanical readout is an analytical challenge that needs standardization. Here, we will provide guidelines for measuring and post-processing of RT-DC data.