Bone marrow niche-mimetics modulate HSPC function via integrin signaling

Kraeter, Martin; Jacobi, Angela; Otto, Oliver; Tietze, Stefanie; Mueller, Katrin; Poitz, David M.; Palm, Sandra; Zinna, Valentina M.; Biehain, Ulrike; Wobus, Manja; Chavakis, Triantafyllos; Werner, Carsten; Guck, Jochen; Bornhauser, Martin

doi:10.1038/s41598-017-02352-5

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Bone marrow niche-mimetics modulate HSPC function via integrin signaling

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Kraeter, M., Jacobi, A., Otto, O., Tietze, S., Mueller, K., Poitz, D. M., et al. (2017). Bone marrow niche-mimetics modulate HSPC function via integrin signaling. SCIENTIFIC REPORTS, 7: 2549. doi:10.1038/s41598-017-02352-5.

Cite as: http://hdl.handle.net/21.11116/0000-0004-B087-C

Abstract

The bone marrow (BM) microenvironment provides critical physical cues for hematopoietic stem and progenitor cell (HSPC) maintenance and fate decision mediated by cell-matrix interactions. However, the mechanisms underlying matrix communication and signal transduction are less well understood. Contrary, stem cell culture is mainly facilitated in suspension cultures. Here, we used bone marrow-mimetic decellularized extracellular matrix (ECM) scaffolds derived from mesenchymal stromal cells (MSCs) to study HSPC-ECM interaction. Seeding freshly isolated HSPCs adherent (AT) and non-adherent (SN) cells were found. We detected enhanced expansion and active migration of AT-cells mediated by ECM incorporated stromal derived factor one. Probing cell mechanics, AT-cells displayed naive cell deformation compared to SN-cells indicating physical recognition of ECM material properties by focal adhesion. Integrin alpha IIb (CD41), alpha V (CD51) and beta 3 (CD61) were found to be induced. Signaling focal contacts via ITG beta 3 were identified to facilitate cell adhesion, migration and mediate ECM-physical cues to modulate HSPC function.