Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

Otto, Oliver; Rosendahl, Philipp; Mietke, Alexander; Golfier, Stefan; Herold, Christoph; Klaue, Daniel; Girardo, Salvatore; Pagliara, Stefano; Ekpenyong, Andrew; Jacobi, Angela; Wobus, Manja; Toepfner, Nicole; Keyser, Ulrich F.; Mansfeld, Joerg; Fischer-Friedrich, Elisabeth; Guck, Jochen

doi:10.1038/nmeth.3281

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Real-time deformability cytometry: on-the-fly cell mechanical phenotyping

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Otto, O., Rosendahl, P., Mietke, A., Golfier, S., Herold, C., Klaue, D., et al. (2015). Real-time deformability cytometry: on-the-fly cell mechanical phenotyping. NATURE METHODS, 12(3), 199-+. doi:10.1038/nmeth.3281.

Cite as: https://hdl.handle.net/21.11116/0000-0004-B0E2-5

Abstract

We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (> 100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.