Monitoring of laser micromanipulated optically trapped cells by digital 
holographic microscopy

Kemper, Bjoern; Langehanenberg, Patrik; Hoeink, Alexander; von Bally, Gert; Wottowah, Falk; Schinkinger, Stefan; Guck, Jochen; Kaes, Josef; Bredebusch, Ilona; Schnekenburger, Juergen; Schuetze, Karin

doi:10.1002/jbio.201000035

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Monitoring of laser micromanipulated optically trapped cells by digital holographic microscopy

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Kemper, B., Langehanenberg, P., Hoeink, A., von Bally, G., Wottowah, F., Schinkinger, S., et al. (2010). Monitoring of laser micromanipulated optically trapped cells by digital holographic microscopy. JOURNAL OF BIOPHOTONICS, 3(7), 425-431. doi:10.1002/jbio.201000035.

Cite as: https://hdl.handle.net/21.11116/0000-0004-B402-E

Abstract

For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be used in combination with microfluidics to observe optically trapped living cells in a minimally invasive fashion during laser micromanipulation. The obtained results demonstrate that DHM multi-focus phase contrast provides label-free quantitative monitoring of optical manipulation with a temporal resolution of a few milliseconds.
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Laser micromanipulation monitoring of optically trapped pancreas tumor cells by quantitative digital holographic phase contrast. The arrows in the false colour coded quantitative phase contrast images indicate the impact of the treatment with focussed laser light.