The optical cell rotator

Kreysing, Moritz K.; Kiessling, Tobias; Fritsch, Anatol; Dietrich, Christian; Guck, Jochen; Kaes, Josef A.

doi:10.1364/OE.16.016984

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The optical cell rotator

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Kreysing, M. K., Kiessling, T., Fritsch, A., Dietrich, C., Guck, J., & Kaes, J. A. (2008). The optical cell rotator. OPTICS EXPRESS, 16(21), 16984-16992. doi:10.1364/OE.16.016984.

Cite as: https://hdl.handle.net/21.11116/0000-0004-B425-7

Abstract

The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In contrast to optical tweezers, OCR uses two counter-propagating divergent laser beams, which are shaped and delivered by optical fibers. The rotation of a trapped specimen is carried out by the rotation of a dual-mode fiber, emitting an asymmetric laser beam. Experiments were performed on human erythrocytes, promyelocytic leukemia cells (HL60), and cell clusters (MCF-7). Since OCR permits the rotation of cells around an axis perpendicular to the optical axis of any microscope and is fully decoupled from imaging optics, it could be a suitable and expedient tool for tomographic microscopy. (C) 2008 Optical Society of America