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Measurement of angstrom to nanometer molecular distances with 19F nuclear spins by EPR/ENDOR spectroscopy.

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Bennati,  M.
Research Group of Electron Paramagnetic Resonance, MPI for Biophysical Chemistry, Max Planck Society;

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Meyer,  A.
Research Group of Electron Paramagnetic Resonance, MPI for Biophysical Chemistry, Max Planck Society;

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Citation

Bennati, M., Meyer, A., Dechert, S., Dey, S., & Höbartner, C. (2020). Measurement of angstrom to nanometer molecular distances with 19F nuclear spins by EPR/ENDOR spectroscopy. Angewandte Chemie International Edition, 59(1), 373-379. doi:10.1002/anie.201908584.


Cite as: http://hdl.handle.net/21.11116/0000-0004-B952-F
Abstract
Spectroscopic and biophysical methods for structural determination at atomic resolution are fundamental in studies of biological function. Here we introduce an approach to measure molecular distances in bio-macromolecules using 19 F nuclear spins and nitroxide radicals in combination with high-frequency (94 GHz/ 3.4 Tesla) electron-nuclear double resonance (ENDOR). The small size and large gyromagnetic ratio of the 19 F label enables to access distances up to about 1.5 nm with accuracy 0.1 - 1 Å. The experiment is not limited by the size of the bio-macromolecule. Performance is illustrated on synthesized, fluorinated model compounds as well as spin-labelled RNA duplexes. The results demonstrate that our simple but strategic spin labelling procedure combined with state-of-the-art spectroscopy accesses a distance range crucial to elucidate active sites of nucleic acids or proteins in solution state.