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Journal Article

Photoactivation of silicon rhodamines via a light-induced protonation

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Frei,  Michelle S.
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

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Johnsson,  Kai
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Frei, M. S., Hoess, P., Lampe, M., Nijmeijer, B., Kueblbeck, M., Ellenberg, J., et al. (2019). Photoactivation of silicon rhodamines via a light-induced protonation. Nature Communications, 10: 4580, pp. 1-10. doi:10.1038/s41467-019-12480-3.


Cite as: http://hdl.handle.net/21.11116/0000-0004-D21D-F
Abstract
hotoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.