The binding of nucleotides and metal ions to elongation factor Tu from Bacillus 
stearothermophilus as studied by equilibrium dialysis

Antonsson, Bruno; Kalbitzer, Hans Robert; Wittinghofer, Alfred

doi:10.1515/bchm2.1981.362.1.735

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The binding of nucleotides and metal ions to elongation factor Tu from Bacillus stearothermophilus as studied by equilibrium dialysis

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Kalbitzer, Hans Robert
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Wittinghofer, Alfred
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Antonsson, B., Kalbitzer, H. R., & Wittinghofer, A. (1981). The binding of nucleotides and metal ions to elongation factor Tu from Bacillus stearothermophilus as studied by equilibrium dialysis. Hoppe-Seyler's Zeitschrift für physiologische Chemie, 362(6), 735-743. doi:10.1515/bchm2.1981.362.1.735.

Cite as: https://hdl.handle.net/21.11116/0000-0004-E67C-E

Abstract

EF-Tu from B. stearothermophilus binds divalent metal ions even in the absence of guanine nucleotides. The association constants necessary for characterizing the multiple equilibria between EF-Tu, GDP and the divalent ions magnesium and manganese were determined by equilibrium dialysis. The constants are 4.6 X 10(4) M-1 and 5.4 X 10(5) M-1 for the binding of Mg2 and 1.0 X 10(5) M-1 and 1.1 X 10(6) M-1 for the binding of Mn2 to EF-Tu and EF-Tu . GDP, respectively. In the absence of divalent ions EF-Tu binds GMP, GDP and GTP with association constants of 3 x 10(3) M-1, 1.7 x 10(7) M-1 and 1.3 x 10(6) M-1, respectively. The binding of GDP in the presence of metal ions is an order of magnitude stronger than in the absence of metal ions.