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Journal Article

Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis

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Hampoelz,  Bernhard
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany;

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Beck,  Martin
Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society;
European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany;
European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Heidelberg, Germany;

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Citation

Hampoelz, B., Schwarz, A., Ronchi, P., Bragulat-Teixidor, H., Tischer, C., Gaspar, I., et al. (2019). Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis. Cell, 179(3), 671-686.e17. doi:10.1016/j.cell.2019.09.022.


Cite as: http://hdl.handle.net/21.11116/0000-0004-F644-A
Abstract
The molecular events that direct nuclear pore complex (NPC) assembly toward nuclear envelopes have been conceptualized in two pathways that occur during mitosis or interphase, respectively. In gametes and embryonic cells, NPCs also occur within stacked cytoplasmic membrane sheets, termed annulate lamellae (AL), which serve as NPC storage for early development. The mechanism of NPC biogenesis at cytoplasmic membranes remains unknown. Here, we show that during Drosophila oogenesis, Nucleoporins condense into different precursor granules that interact and progress into NPCs. Nup358 is a key player that condenses into NPC assembly platforms while its mRNA localizes to their surface in a translation-dependent manner. In concert, Microtubule-dependent transport, the small GTPase Ran and nuclear transport receptors regulate NPC biogenesis in oocytes. We delineate a non-canonical NPC assembly mechanism that relies on Nucleoporin condensates and occurs away from the nucleus under conditions of cell cycle arrest.