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Journal Article

A facile oxygen-17 NMR method to determine effective viscosity in dilute, molecularly crowded and confined aqueous media.

MPS-Authors
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Becker,  S.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Griesinger,  C.
Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Rezaei-Ghaleh, N., Munari, F., Becker, S., Assfalg, M., & Griesinger, C. (2019). A facile oxygen-17 NMR method to determine effective viscosity in dilute, molecularly crowded and confined aqueous media. Chemical Communications, 55(82), 12404-12407. doi:10.1039/c9cc06124j.


Cite as: http://hdl.handle.net/21.11116/0000-0005-0B1E-F
Abstract
We present an NMR method based on natural abundance 17O relaxation of water to determine effective viscosity in biological aqueous samples. The method accurately captures viscosity of dilute and crowded protein solutions and offers a fairly simple way to quantify the internal fluidity of biological condensates formed through phase separation.