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Journal Article

Investigations about structure, distribution and dynamics of different organic substrates in the DOM of lake Plußsee


Münster,  Uwe
Department Microbial Ecology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Münster, U. (1985). Investigations about structure, distribution and dynamics of different organic substrates in the DOM of lake Plußsee. Archiv für Hydrobiologie, Suppl. 70(4), 429-480.

Cite as: http://hdl.handle.net/21.11116/0000-0005-0BAB-F
Adsorption of the DOM on XAD-2, PVP and Polyamide-6 at different pH was studied. Adsorption was significant pH depending. Polyphenols in the adsorbed fractions were determined by GLC. OH and CO2H groups preferentially in ortho- and meta-position were predominate and developed hydrogen bridge bonding to proteins. By this mechanism polyphenol humic matter and labile substrates (proteins and carbohydrates) coexist in a same molecular matrix. Fractionation experiments with Sephadex, PAGE and IEF revealed a complex structure in the DOM with varying molecular weight pattern. Three major fractions were found with different subunits. Molecular weights ranged between 1,500 to 90,000 Dalton. The high molecular weight substrates are stratified and corresponded with the autotrophic and heterotrophic activity in their dynamics and distribution. Humic substrates contained acidic groups with IEPs between pH 2-3. Polyphenols, proteins and acidic glycoproteins were associated in these fractions. These humic substrates showed high free electrophoretic mobility in the PAGE experiments. Flexible structure units were confirmed. Free dissolved monomeric carbohydrates existed in only low steady state concentrations. Bound polymeric carbohydrates showed 10 to 100 times higher concentrations and significant gradients that corresponded with autotrophic and heterotrophic activities.