Enzymatic synthesis of bacteriophage fd viral DNA

Meyer, Thomas F.; Geider, Klaus

doi:10.1038/296828a0

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Enzymatic synthesis of bacteriophage fd viral DNA

MPS-Authors

/persons/resource/persons82047

Meyer, Thomas F.
Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society;

/persons/resource/persons93045

Geider, Klaus
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

https://www.nature.com/articles/296828a0.pdf
(Any fulltext)

https://doi.org/10.1038/296828a0
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Meyer, T. F., & Geider, K. (1982). Enzymatic synthesis of bacteriophage fd viral DNA. Nature, 296(5860), 828-832. doi:10.1038/296828a0.

Cite as: https://hdl.handle.net/21.11116/0000-0005-3AC4-D

Abstract

An enzyme system with requirements similar to those for replication of phage fd replicative form (RF) DNA in bacteriophage fd-infected cells has been reconstituted with purified fd gene 2 protein, and DNA polymerase III holoenzyme, DNA binding protein I and rep-protein (rep-helicase) of Escherichia coli. The system generates viral circular single strands, which are infective for E. coli spheroplasts. Parental and newly synthesized DNA are covalently connected in early stages of replication, as expected for DNA replication using the rolling circle mechanism. Single-stranded tails of the rolling circle intermediates are cleaved after a full round of replication by gene 2 protein and circularized by the same enzyme molecule.