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Can mixed cultures help the understanding of natural heterotrophic processes?

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Höfle,  Manfred G.
Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Höfle, M. G. (1984). Can mixed cultures help the understanding of natural heterotrophic processes? Ergebnisse der Limnologie/Advances in Limnology, 19, 53-58.


Cite as: http://hdl.handle.net/21.11116/0000-0005-538C-0
Abstract
Batch cultures of natural bacterial communities were grown in filter-sterilized seawater from coastal and open-ocean sites. Natural coastal seawater was supplemented with up to 500 μg/l of various nitrogen containing substrates like putrescine, cadaverine, glutamic acid, serine and ammonia. The removal of these compounds was monitored by high performance liquid chromatography. Increases in bacterial cell number during the batch culture were counted with an epifluorescence microscope after acridine organe staining (AODC). All compounds were removed from coastal seawater cultures within 48 hours with no corresponding increase in bacterial yield or growth rate relative to control (unsupplemented) cultures. Shipboard experiments with open-ocean deep water (1500 m) showed similar results, but slower removal of the added substrates from seawater. Indicators of metabolic activity such as glucose and glutamic acid uptake and mineralization potentials per cell and ratio of colony forming units (CFU) to total cell counts (AODC) increased markedly during the growth phase. This mixed culture approach could therefore help estimate heterotrophic potentials of compounds that are difficult to degrade over reasonable incubation times with natural bacterial communities and also elucidate the biochemical fate of these compounds.